T cell development, regulation and plasticity,

and their role in generating protective immunity.

 


On this website you can find news & updates about m14955944_10154374138762599_4126081871636820589_n(1)y current research projects (incl. talks, publications  etc.), the translation of these findings to the public and how I engage in improving my skill set and those of others. In addition, you can find all my prior research projects, publications, conference talks, positions and more.
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Research focus

The current concept of a relative homogeneous and quiescent naïve T cell population is in desperate need of revision (ref.), as recent insights have shown great diversity in both phenotype and function within the naïve T cell compartment. This new understanding has major impact on current thinking and experimentation, including but not limited to homeostasis, reconstitution, immunotherapy, and aging, in the field of naïve T cell immunology.Logo extra

How this heterogeneity of naive T cells contributes to providing protective immunity towards infection, auto reactivity and malignancy is unknown. In the cases of infection and autoimmune disease, the generation of (auto-) antibodies by B cells is of great importance. How T cell dependent B cell activation is altered and how it can be utilized as therapeutic targets in the light of new-found naive T cell heterogeneity is to be determined.

My research addresses conceptual and mechanistic immunological questions in T cell immunology with a strong translational aspect, utilizing murine models and studying human health and disease. More details about my research can be found here.

 


Current research projects:

  • How, where and when do B cells lose tolerance and acquire different self-reactivities (epitope spreading)?
  • Does the Germinal Center (GC) response differ between the different initiators ?
  • How does successful immune therapy with a microbiome-derived peptide alter the T cell landscape in rheumatoid arthritis patients?
  • How is naive T cell development, regulation and plasticity altered in health, ageing and chronic inflammation ?

 


Utilizing state of the art techniques:

Confocal microscopy, multi-photon intravital microscopy, single cell RNAsequencing (10X), Flow Cytometry and Cell sorting, with (customized) functional assays, among  others.

Picture1
Confocal image of a whole murine spleen that highlights the Germinal Center (blue, GL7) within a follicle characterized by marginal zone macrophages (green, CD169), extra-follicular located plasma cells (white, CD138).
10X zoom, stitched image (~100 tiles)

 

 

 

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2-photon microscopy image of 1 section through a lymph node.

Left: a portion of cells in the Germinal Center got a different color at the start of experiment. Colors are by endogenous fluorescence via Confetti reporter
Right: 2 germinal centers (red & green) seen at a later time-point (~several days-weeks). Similar color means ‘clonal expansion’ of those cells.
(white, auto-fluorescence)